Figure 3.

Ablation of ATF3, but not pSTAT3, induction by leptin in Atf3^LepRbKO mice. A: We crossed Lepr^cre mice onto the Atf3^flox background to generate Lepr^cre/cre;Atf3^flox/flox (Atf3^LepRbKO) mice and littermate controls. B and C: Control (B) and Atf3^LepRbKO (C) mice were fasted for 4 h prior to treatment with leptin (5 mg/kg i.p.) followed by perfusion 6 h later and processing for the IHC detection of ATF3 (black nuclei). D and E: Control (D) and Atf3^LepRbKO (E) mice were fasted overnight before treatment with leptin (5 mg/kg i.p.) 1 h before perfusion and processing for the IHC detection of pSTAT3 (black nuclei). B–E: Digital zoom of the boxed area is shown in the upper right corner.