Table 1.

Genes coordinately regulated by leptin across multiple conditions in hypothalamic LepRb neurons

Gene	Expression (FPKM), WT PBS	Enrichment (fold), WT PBS	Regulation (fold)
			WT 3-h Lep vs. WT PBS	WT 10-h Lep vs. WT PBS	Ob PBS vs. WT PBS	Ob 10-h Lep vs. Ob PBS
Serpina3i	1.08	11.67	1.89	2.48	0.13	10.46
Socs3	3.15	2.89	5.60	3.14	0.25	10.00
Serpina3h	3.55	5.83	2.97	6.15	0.32	7.33
Traf3ip3	1.15	2.83	1.61	2.36	0.53	1.91
Irgm2	0.79	0.90	1.56	1.77	0.59	1.71
Prokr2	1.12	1.26	1.94	2.29	0.61	1.51
Serpina3c	0.76	7.70	1.04	2.77	0.20	9.92
Serpina3n	90.24	8.14	1.04	2.06	0.26	5.06
Irf9	9.23	2.05	1.38	1.83	0.38	3.01
Bcl3	1.34	2.42	1.48	1.75	0.43	1.54
Vwa5a	7.08	2.33	1.23	2.01	0.52	1.63
Serpina3m	2.93	7.67	1.38	3.92	0.52	2.79
Stat3	37.86	2.48	1.44	1.62	0.52	2.33
Asb4	69.15	7.10	1.30	1.74	0.61	1.64
Vwce	0.20	1.27	1.01	0.59	2.53	0.61
Rasl11a	12.01	0.96	1.80	1.57	0.74	1.83
Junb	31.52	2.02	1.76	1.91	0.97	1.67
Atf3	2.81	4.35	5.82	4.58	1.02	2.39
Ly6a	4.95	1.40	0.57	0.45	1.67	0.79
Vwf	0.45	2.52	0.52	0.23	1.69	0.76
Acer2	0.86	1.20	0.66	0.44	1.72	0.67
Drd1a	2.30	0.74	1.63	0.72	0.66	1.66
1700024P16Rik	0.11	0.78	0.59	1.44	8.42	0.43

Shown are the LepRb-enriched transcripts that were significantly different for at least three of the comparisons listed and for which increased leptin promoted changes in the same direction (and/or leptin deficiency promoted opposite changes). Expression (in fragments per kilobase per million, FPKM) and enrichment (fold relative to supernatant) are shown for the wild-type vehicle-treated (WT PBS) condition. Note that while not all transcripts shown were enriched in this condition, all were enriched in LepRb neurons in at least one condition. Also shown is the fold change in expression across the listed conditions/comparisons. Lep = leptin, 5 mg/kg i.p. for the indicated time; Ob = Lep^ob/ob mice. Bolded values are statistically significant between conditions.