Table 2.
Increased sensitivity for detecting changes in LepRb neuron gene expression in TRAP relative to whole hypothalamic samples
| Gene | Fold change, TRAP | Fold change, whole hypothalamus |
|---|---|---|
| Socs3 | 12.51 | 6.02 |
| Atf3 | 18.90 | 2.40 |
| JunB | 4.49 | 1.29 |
| Arid5a | 10.67 | 1.10 |
| Etv6 | 3.48 | 1.18 |
We performed qPCR to measure changes in gene expression following 3 h of leptin treatment (5 mg/kg i.p.) compared with vehicle in LepRb neuron–derived TRAP material or RNA derived from whole hypothalamic lysates. Bolded numbers represent statistically significant changes in gene expression (unpaired t test, P < 0.05).