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. 2018 Apr 17;46(9):4677–4688. doi: 10.1093/nar/gky264

Figure 3.

Figure 3.

The HDR and NHEJ activities of gRNAs of different lengths combined with Cas9 in HEK293T cells. The design of the gRNAs with different lengths for RBM20-2, RBM20-3, RBM20-g2, GRN-1, GRN-2, GRN-g1, ATP7B-1, ATP7B-g2 and ATP7B-g5. The genomic sequences are shown above the gRNA series. The gRNA series of RBM20-2 and GRN-2 included ones with extra nucleotides of the endogenous genomic sequence or non-guanine extensions. Extra nucleotides that match and do not match the endogenous sequences are highlighted in green and blue, respectively. The frequencies of the HDR alleles (red) and the NHEJ alleles (blue) among the total alleles ± S.E. induced by WT Cas9 and these gRNA series are shown (n = 3). Student's t-test was used to evaluate the difference in the HDR and NHEJ activities between the original gRNA and the modified gRNAs. *P< 0.05 and **P< 0.01.