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. 2018 May 14;11:147. doi: 10.3389/fnmol.2018.00147

FIGURE 1.

FIGURE 1

Re-sort analysis, immunostaining, and q-PCR of flow-sorted Lgr5+ and Lgr6+ cells from the postnatal cochlea. (A) At P3, Lgr5 was expressed in the third row of Deiters’ cells (DC3), the inner pillar cells (IPs), the inner phalangeal cells (IPCs), and the lateral GER, while Lgr6 was only expressed in the IPs. (B) Cryosection showed that Lgr5 was expressed in DC3s, IPs, IPCs and the GER, and Lgr6 was only expressed in a subset of IPs in the P3 organ of Corti. (C) GFP+ cells and GFP– cells were isolated using flow cytometry. Re-sort analysis of GFP+ cells demonstrated > 90% purity. (D) Immunostaining of Lgr5+ cells and Lgr6+ cells from the cochlea showed a high percentage of Sox2+ (95.4% and 95.2%, respectively) and GFP+ (95.8% and 96.6%, respectively) cells, and no Myo7a+ cells, among the sorted cells. (E,F) q-PCR showed that isolated Lgr5+ cells and Lgr6+ cells had significantly higher Lgr5 and Lgr6 expression, slightly higher Sox2 expression, and significantly lower Brn3.1 expression compared to the Lgr5- cells and Lgr6– cells, respectively. Scale bars are 20 μm. ∗∗p < 0.01.