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. 2018 May 14;11:147. doi: 10.3389/fnmol.2018.00147

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Copyright © 2018 Zhang, Guo, Lu, Cheng, Sun, Li, Zhao, Lai, Zhang, Yu, Tang, Chen, Chai and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Lgr6+ progenitors generate more HCs compared to Lgr5+ progenitors in vitro. (A) Schematic depicting the experimental process. We dissected the cochleae and then cultured the sorted GFP+ cells at 20 cells/μl for 10 days. (B) Both Lgr5+ and Lgr6+ progenitors could generate Myo7a+ colonies. (C) Both Lgr5+ and Lgr6+ progenitors could generate Myo7a+/EdU+ cells inside of the colonies. (D) Both Lgr5+ and Lgr6+ progenitors could generate Myo7a+ cells outside the colonies. (E) The total number of Myo7a+ cells in each well per 2,000 cells and the numbers of Myo7a+ cells inside and outside the colonies. The Lgr5+ progenitors formed around 140 Myo7a+ cells per 2,000 cells and about 33 Myo7a+ cells inside the colonies, while the Lgr6+ progenitors formed around 360 Myo7a+ cells per 2,000 cells and about 36 Myo7a+ cells inside the colonies. (F) The numbers of colonies in each well per 2,000 cells. The Lgr5+ progenitors formed around 20 colonies and 10 Myo7a+ colonies, while the Lgr6+ progenitors formed around 13 colonies and 6 Myo7a+ colonies. (G) Lgr5+ progenitors generated significantly more Myo7a+/EdU+ cells than Lgr5+ progenitors inside of the colonies (11.0 ± 0.91 and 1.5 ± 0.29 HCs, respectively). (H) Each Myo7a+ colony from the Lgr6+ progenitors generated significantly more HCs than the Myo7a+ colonies from Lgr5+ progenitors. ^∗∗p < 0.01, n = 4. Scale bars are 20 μm.