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. 2018 May 14;11:147. doi: 10.3389/fnmol.2018.00147

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Copyright © 2018 Zhang, Guo, Lu, Cheng, Sun, Li, Zhao, Lai, Zhang, Yu, Tang, Chen, Chai and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Lgr5+ progenitors have greater sphere-forming ability than Lgr6+ progenitors, and Lgr6+ progenitors have greater differentiation ability than Lgr5+ progenitors (A) Schematic depicting the experimental procedure. Cochleae were dissected, and flow-sorted Lgr5+ and Lgr6+ progenitors were cultured for 5 days in ultra low-attachment dishes, after which the spheres were transferred to laminin/polylysine (1:1)-coated 4-well dishes and cultured for another 10 days with EdU added to the medium from day 4 to day 8. (B) Both Lgr5+ and Lgr6+ progenitors could generate spheres. (C) Lgr5+ progenitors generated significantly more spheres than Lgr6+ progenitors, and spheres from Lgr5+ progenitors had a significantly larger diameter than those from the Lgr6+ progenitors. (D) Differentiated spheres from Lgr5+ progenitors generated Myo7a+ HCs and Myo7a+/EdU+ double-positive cells (arrows). Differentiated spheres from Lgr6+ progenitors also generated Myo7a+ HCs. (E) Lgr5+ progenitors generated significantly more Myo7a+ spheres than those from Lgr6+ progenitors. (F) Each differentiated sphere from Lgr6+ progenitors generated significantly more Myo7a+ HCs than those from Lgr5+ progenitors. (G) The 2,000 differentiated spheres from Lgr6+ progenitors generated significantly more Myo7a+ HCs than those from Lgr5+ progenitors. ^∗p < 0.05; ^∗∗p < 0.01, n = 4. Scale bars are 50 μm.