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The presence of 2 mol% GWALP23 does not change phase boundaries of DSPC/DOPC/chol at high cholesterol fraction. FRET from DHE to FAST DiO is shown along the trajectory in the inset in the absence (black, upper) and in the presence of 1 mol% GWALP23 (gray, shifted lower for clarity). Boundaries correspond to the shaded regions. The width of each shaded region is the fitting error.
