Bending moduli of Ld and Lo phases in lipid-only mixtures. (A) The compositions we compared are shown representatively as line endpoints on the generic phase diagram above. Simplified, lower-tieline, and high-cholesterol Ld and Lo compositions for bSM/POPC/chol, bSM/DOPC/chol, DSPC/POPC/chol, and DSPC/DOPC/chol are plotted in Fig. S1. The difference in bending moduli, Δκ = κLo – κLd, between simple, lower-tieline, and high-cholesterol Lo and Ld phases for bSM/POPC/chol (A), bSM/DOPC/chol (B), DSPC/POPC/chol (C), and DSPC/DOPC/chol (D) is plotted with respect to distance in composition space or the compositional separation of Ld and Lo phases.