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. 2018 May 8;114(9):2095–2106. doi: 10.1016/j.bpj.2018.03.029

Figure 7.

Figure 7

Exemplary single-channel traces of recombinant CaV1.2 calcium channel isoforms. HEK 293 cells were transiently transfected with Cav1.2, α2δ1, β2a, and GFP in equal molar ratios. An illustration of the applied pulse protocol (150-ms pulse length, HP of −100 mV, test pulse to +10 mV, interpulse time 1 Hz) is shown. (A) Ten representative consecutive traces of at least 180 recorded sweeps per experiment are highlighted for each channel isoform. (B) The mean ensemble average current derived from 180 traces is shown. Scale bar is given in pA for single-channel events and in fA for averaged traces.