Table 2.
Average Transfer Energies of the ALPS Motif of ArfGAP1 and Its Mutants to 15% Anionic Lipid Vesicles of Different Curvature
| Radius, nm | Wild-Type |
2Ki |
2Kt |
4Ki |
4Ki/4Et |
|||||
|---|---|---|---|---|---|---|---|---|---|---|
| Cal. | Exp. | Cal. | Exp. | Cal. | Exp. | Cal. | Exp. | Cal. | Exp. | |
| 40 | −9.3 (1.0) | −5.5 | −9.4 (1.6) | −6.8 | −9.8 (0.7) | −5.7 | −11.6 (0.2) | −5.4 | −8.7 (0.3) | −5.3 |
| 90 | −8.2 (0.4) | −3.4 | −9.4 (0.5) | −6.8 | −9.0 (0.4) | −4.2 | −10.9 (0.3) | −4.6 | −8.5 (0.5) | −4.7 |
| 120 | −8.0 (0.2) | −3.3 | −9.6 (0.5) | −6.8 | −9.1 (0.2) | −3.6 | −10.8 (0.3) | −4.2 | −7.9 (1.0) | −4.3 |
The numbers in parentheses indicate SD. Experimental binding free energies of arfgap1 were obtained from floatation assay (27). The given % protein bound information in the article was converted into binding free energy using the method described in the Supporting Material.