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. 2018 May 15;11:2805–2813. doi: 10.2147/OTT.S162381

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© 2018 Ren et al. This work is published and licensed by Dove Medical Press Limited

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Representative analyses of DAPK1 and MGMT methylation in CMGTs.

Notes: (A) The methylation status of DAPK1 and MGMT genes was measured in CHMm and CHMp cells via MSP assay (M, methylated primer; U, unmethylated primer). (B) The MSP results of DAPK1 and MGMT genes in part of blood and tissue samples (M, methylated primer; U, unmethylated primer). Methylated group (malignant tumor group): 4, 12, 13, 14, 15, 16, 17, 18, 19; unmethylated group (benign tumor group): 9, 10; partly methylated group: 1, 2, 3, 5, 6, 7, 8, 11, 20, 21, 22, 23, 24; normal group: 25, 26, 27, 28, 29, 30.

Abbreviations: CMGTs, canine mammary gland tumors; MGMT, O⁶-methylguanine-DNA methyltransferase; MSP, methylation-specific polymerase chain reaction.