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Methylated specific primer sequences used for MSP
| Gene | Forward primer | Reverse primer | Annealing temperature/time | Amplicon |
|---|---|---|---|---|
| DAPK1 | AAGGAAGAGGGAGAGTCGTC | CGACGAAAAACTCGACAAAT | 58°C/30 s | 128 bp |
| MGMT | AGGGAGAGAATGTTTTTACGC | AACGATAAAAACCTCGACTACG | 60°C/30 s | 159 bp |
Abbreviation: MSP, methylation-specific polymerase chain reaction.
