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Sequences of qRT-PCR primers
| No | Gene | Forward primer | Reverse primer | Annealing temperature/time | Amplified fragments |
|---|---|---|---|---|---|
| NM001197157.1 | DAPK1 | GCTCATGTCTCTGCAGCAGTTTG | ACACATCCTGGACCGTTTCACTC | 60°C/30 s | 146 bp |
| NM001003376.1 | MGMT | CGCGATTCACCATCCCATT | AACAATAAAAACCTCAACTACAAAC | 60°C/30 s | 85 bp |
| Z_70044 | β-actin | GCTGTCCTGTCCCTGTATTCC | AGCCAAGTCCAGACGACGCAAG | 58°C/30 s | 132 bp |
Abbreviation: qRT-PCR, quantitative real-time polymerase chain reaction.
