Figure 1.

Overview of the working principle of ERISM. (a) A microcavity with a cell attached to the functionalized top gold layer is depicted. (b) The microcavity is illuminated from underneath, and the reflectivity is recorded by an sCMOS camera. (c) In parallel, a dia-illumination phase-contrast image is recorded on a separate CMOS camera using a different spectral channel. Shown is the image obtained for a microcavity on which 3t3 fibroblast cells are cultured. (d) Representative reflectance images for illumination at 647, 650, and 653 nm and reflectivity versus wavelength for a single pixel from the recorded image stack are shown. (e) A schematic illustration of the ERISM image analysis process is given. Using the reflection spectra extracted from the image stack for each pixel, the “ERISM-Calc” software derives the cell-induced vertical displacement. Data are shown for the same cells shown in (c) and (d). All scale bars, 50 μm. To see this figure in color, go online.