Figure 4.

GO induces ROS generation in MG-63 and K₇M₂ cells.

Notes: (A) MG-63 and K₇M₂ cells were treated with 0, 25, and 50 µg/mL GO for 2 h. Then, DHE analyses were applied to detect ROS generation. (B) Fluorescence analysis of ROS content. MG-63 and K₇M₂ cells were treated with 0, 25, and 50 µg/mL GO for 0.5, 2, 4, and 8 h. Fluorescence intensity was calculated compared to untreated control cells. Values are presented as mean±SD and compared with untreated control (*P<0.05, ^#P<0.01). (C) Cell viability assay was conducted by MTT. MG-63 and K₇M₂ cells were treated with 0, 25, and 50 µg/mL GO with or without NAC (10 mmol/L) for 8 h. ^#P<0.01 (compared with GO treatment alone).

Abbreviations: DHE, dihydroethidium; GO, graphene oxide; NAC, N-acetylcysteine; ROS, reactive oxygen species.