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. 2018 May 17;13:2907–2919. doi: 10.2147/IJN.S159388

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© 2018 Tang et al. This work is published and licensed by Dove Medical Press Limited

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GO induces Nrf-2 transport from the cytoplasm to the nucleus in MG-63 cells.

Notes: (A) MG-63 and K₇M₂ cells were treated with or without GO (25 µg/mL) for 2 h. Nrf-2 primary antibody and a rabbit IgG-CY3 secondary antibody were added for immunofluorescence assay (×40). (B) Cytoplasmic and nuclear Nrf-2 were determined by Western blot analysis with reference to β-actin and lamin B1. MG-63 and K₇M₂ cells were stimulated with or without GO (25 µg/mL) for 2 h. Cytoplasmic and nuclear cells were isolated by ER Nuclear and Cytoplasmic Extraction Reagents. (C) Fluorescence analysis of ROS content by DHE. Cells were treated with 0, 25, and 50 µg/mL GO with or without ML385 (2 µmol/L) for 2 and 4 h. ^#P<0.01 (compared with GO treatment alone).

Abbreviations: DHE, dihydroethidium; DAPI, 4,6-diamidino-2-phenyl-indole; GO, graphene oxide; ROS, reactive oxygen species.

GO induces Nrf-2 transport from the cytoplasm to the nucleus in MG-63 cells.

Notes: (A) MG-63 and K₇M₂ cells were treated with or without GO (25 µg/mL) for 2 h. Nrf-2 primary antibody and a rabbit IgG-CY3 secondary antibody were added for immunofluorescence assay (×40). (B) Cytoplasmic and nuclear Nrf-2 were determined by Western blot analysis with reference to β-actin and lamin B1. MG-63 and K₇M₂ cells were stimulated with or without GO (25 µg/mL) for 2 h. Cytoplasmic and nuclear cells were isolated by ER Nuclear and Cytoplasmic Extraction Reagents. (C) Fluorescence analysis of ROS content by DHE. Cells were treated with 0, 25, and 50 µg/mL GO with or without ML385 (2 µmol/L) for 2 and 4 h. ^#P<0.01 (compared with GO treatment alone).

Abbreviations: DHE, dihydroethidium; DAPI, 4,6-diamidino-2-phenyl-indole; GO, graphene oxide; ROS, reactive oxygen species.