Figure 6.

GO induces apoptosis in K₇M₂ cancer cells.

Notes: (A) K₇M₂ cells were exposed to 0, 25, and 50 µg/mL GO for 4 and 8 h. Annexin V-FITC Apoptosis Detection Kit was used to determine the percent of early apoptosis (VA%) and late apoptosis cells (NVA%). The experiment was repeated three times and one representative experiment is shown, and each value represents the mean±SD of three in dependent experiments and compared with untreated control (^#P<0.01). (B) GO induces apoptosis in K₇M₂ cancer cells, which were treated with 25 and 50 µg/mL GO for 2, 4, and 8 h. Bcl-2, caspase-3, and cleaved-caspase-3 were processed at all concentration points studied.

Abbreviations: GO, graphene oxide; NVA, nonviable apoptosis cell; PI, propidium iodide; VA, viable apoptosis cell.