Figure 7.
GO stimulates autophagy in MG-63 cancer cells.
Notes: (A) MG-63 and K7M2 cells were exposed to 0, 25, 50 µg/mL GO for 2 h, respectively. Autophagy-related punctate structures in the cells were dyed by MDC solution and examined under a fluorescence microscope at ×40 magnification. Yellow arrows: autophagosomes. (B) MG-63 cells were stimulated with 25 and 50 µg/mL GO for 2, 4, and 8 h. The ratios of cytosolic LC3-I and lipidated autophagosome-bound LC3-II, ATG5, ATG3, and ATG7 were determined by Western blot. (C) To test the autophagic flux, MG-63 cells were stimulated with 25 µg/mL GO for 8 h and 100 nmol/L Baf was added 4 h before the cells were harvested.
Abbreviations: Baf, bafilomycin A1; GO, graphene oxide; MDC, dansylcadaverine.