Figure 1.

(a) Example of Golph4 (red, Golgi), Erg (green, nucleus), and ICAM2 (blue, luminal surface) stainings of a subset of a P6 mouse retinal plexus. (b) A binary mask generated from the ICAM2 staining in (a) is shown. Note that the hemodynamics recovered in the blue highlighted region will not be accurate because of the missing connections to the nearby vessels. The interested reader can refer to (5, 6, 14) for analyses based on larger plexuses. (c) Results of the skeletonisation step are shown. (d) A flow simulation setup defines the inlet, outlet, and seed position.