Figure 4.

(A) Comparisons of the fraction of native contacts, Q, formed during folding and misfolding LBTs. For the indicated variants with N total LBTs, histograms were calculated using all frames from all N trajectories. The data for Z (E342K, N = 58) and S (E264V, N = 35) are in purple and blue, respectively. Suppressor variants with mutations at residues 290 and/or 342 are Glu/Glu (K290E/E342, N = 25), Glu/Lys (K290E/E342K, N = 40), and Ser/Lys (K290S/E342K, N = 48) in orange, gray, and pink, respectively. In all of the histograms, the WT (N = 285) data are in green, and darker-colored regions represent the overlap between WT and mutant distributions. (B) A close-up view shows the network of electrostatic and hydrogen-bonding interactions formed by Glu342 in WT A1AT.