Figure 1. FIGURE 1: Frequent adaptive regrowth of snf1∆ strains during chronological aging under caloric restriction (CR- 0.5% glucose) conditions.

(A) Qualitative CLS assay showing the rapid loss of viability by a snf1∆ strain (JS1394) compared to WT (JS1256) as the cells chronologically age in expired SC NR (2% glucose) medium.

(B) Reactive oxygen species (ROS) accumulation in snf1∆ strains grown into stationary phase during CR culture conditions, as indicated by dihydrorhodamine 123 (DHR) staining.

(C) CAN1 gene mutation frequency in WT and snf1∆ strains grown under NR and CR conditions.