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. Author manuscript; available in PMC: 2018 May 21.
Published in final edited form as: J Thromb Haemost. 2012 May;10(5):940–950. doi: 10.1111/j.1538-7836.2012.04675.x

Figure 1.

Figure 1

Figure 1

mADAMTS13 digestion of full-length mVWF and mVWF115. Recombinant mADAMTS13 digests were performed as outlined under methods. (A) Varying concentrations of mADAMTS13 were incubated with 1 U/mL full-length mVWF for 24 h with 1.5 M Urea. Multimer graphs for wild-type, R1597W and Y1605A/M1606A mouse VWF were plotted using a four-parameter curve fit. The concentration of ADAMTS13 required to cause a 50% loss of multimer height was determined. Symbols are representative of two independent experiments. (B) Comparison of the mADAMTS13 concentrations necessary for 50% loss of intact mVWF115. Varying mADAMTS13 concentrations were incubated with mVWF115 for 4 h under non-denaturing conditions. The assay was run four times for each construct. Bars represent the mean values.