Mouse VWF antigen levels after hydrodynamic injection. VWF knockout mice expressing wild-type, R1597W or Y1605A/M1606A mVWF were sampled after hydrodynamic injection (n ‡ 10). VWF:Ag levels were determined viaELISA. (A) VWF:Aglevels from day 2 to 14 after hydrodynamic injection. Symbols represent means with SEM error bars. (B) VWF:Ag levels from day 14 to 42 after hydrodynamic injection. R1597Wwas statistically lower from wild type via paired t-test. (C) Data by day for days 14, 21, 28 and 35. Each circle represents data from a single mouse. No statistical differences were found with the exceptions of wild type vs. R1597 Won days 21 and 28, using one-way ANOVA, Tukeys post test.