Figure 6.
Thrombogenesis in response to ferric chloride injury. VWF knockout mice expressing only Vwf cDNA after hydrodynamic injection with VWF:Ag levels between 0.5 and 1.7 U mL) 1 were evaluated using 10% ferric chloride injury to the cremaster. (A) Time to a stable occlusive thrombus. Each circle represents an individual mouse, bars represent median occlusion time. The experiment was stopped at 40 min; mice that failed to occlude were recorded as 40 min. (B) Platelet accumulation via mean accumulated fluorescence. Mean with SEM was graphed for each mutation. *P < 0.05 vs. wild type and vs. R1597W. (C) Total platelet accumulation. Area under the curve was determined. Each circle represents a single mouse, bars indicate mean.