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. 2018 May 21;13(5):e0197664. doi: 10.1371/journal.pone.0197664

Fig 5. Rosmarinic acid derivatives also inhibit ε-Pol binding.

Fig 5

(A) Western blot analysis for Pol pulled-down by ε-biotin in the presence of the indicated compounds. Arrows: 3×FLAG-Pol. (B) ssDNA-biotin was pulled-down in the presence of the indicated compounds, and detected by EtBr staining. Arrow: 71-base ssDNA. (C) dsRNA-RIG-I EMSA in the presence of the indicated compounds (100 μM). *: monomeric dsRNA, **: the dsRNA-RIG-I complex. (D) Hep38.7-Tet cells were treated with 10 μM rosmarinic acid, compounds 12c, 13c, 2, and 21a, 100 nM lamivudine, or 400 ng/ml tetracycline. Extracellular HBV DNA and cell viability were measured as in (Fig 3A and 3B). Data are from one representative of at least two independent experiments; the means and S.D. of triplicate experiments are shown (* p < 0.05, ** p < 0.01, *** p < 0.001). (E) A Western blot analysis for Pol pulled-down by ε-biotin in the presence of the indicated compounds. Arrows: 3×FLAG-Pol. Spliced images are indicated with a dividing line.