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. 2018 May 9;14(5):e1007054. doi: 10.1371/journal.ppat.1007054

Fig 3. Parameters influencing salubrinal-induced fusion.

Fig 3

(A) Vero cells were infected with strains KOS, F, or 17 at MOIs of 0.5, 1, or 3 and were incubated in medium containing 50 μM salubrinal. At 12 hpi, the fusion ratio (% free nuclei) was determined by flow cytometry. The mean values (±SD) from 3 independent experiments are plotted. (B) After infection with strain 17 (MOI = 3), cells were incubated in medium containing DMSO, and 50 μM salubrinal was added for the indicated time intervals. After a total of 12 hpi, the fusion ratios were measured by flow cytometry. Data are from 2 independent experiments. A student T-test was used to determine statistical significance for samples compared to the DMSO control. (C) After infection with strain 17 (MOI = 3), cells were incubated for the indicated time periods in medium containing 50 μM salubrinal, which was then replaced with control medium. At 12 hpi, the fusion ratio was determined by flow cytometry, and the data were analyzed as in (3B).