Table 1. Neutralizing antibody titers and anti-RVFV total IgG adjusted SumOD ELISA results of plasma from vaccinated and control animals.
Inoculum | rZH501-ΔNSs | rZH501-ΔNSs-ΔNSm | Sham Inoculated Controls | ||||||
---|---|---|---|---|---|---|---|---|---|
Mean PRNT50 ± STDEV | Mean PRNT80 ± STDEV | Anti-RVFV IgG mean SumOD ± STDEV | Mean PRNT50 ± STDEV | Mean PRNT80 ± STDEV | Anti-RVFV IgG mean SumOD ± STDEV | Mean PRNT50 ± STDEV | Mean PRNT80 ± STDEV | Anti-RVFV IgG mean SumOD ± STDEV | |
Day 7 post-vaccination | 1:867 ± 393 | 1:<400 ± 0 | 0.004 ± 0.006 | 1:<400 ± 0 | 1:<400 ± 0 | -0.005 ± 0.0477 | 1:<400 ± 0 | 1:<400 ± 0 | 0.008 ± 0.023 |
Day 14 post-vaccination | 1:23,467 ± 14,964 | 1:3,533 ± 4,709 | 0.111 ± 0.105 | 1:10,933 ± 4,572 | 1:933 ± 547 | 0.0741 ± 0.045 | 1:<400 ± 0 | 1:<400 ± 0 | -0.002 ± 0.013 |
Day 21 post-vaccination | 1:59,733 ± 34,976 | 1:8,267 ± 9,407 | 0.382 ± 0.125 | 1:26,667 ± 20,001 | 1:6,400 ± 3,505 | 0.258 ± 0.193 | 1:<400 ± 0 | 1:<400 ± 0 | -0.003 ± 0.007 |
Day 28 post-vaccination | 1:29,867 ± 17,488 | 1:13,867 ± 6,292 | 0.689 ± 0.168 | 1:10,733 ± 8,647 | 1:3,000 ± 2,814 | 0.384 ± 0.225 | 1:<400 ± 0 | 1:<400 ± 0 | 0.009 ± 0.004 |
Day 35 post-vaccination | 1:25,707 ± 15,989 | 1:7,333 ± 9,253 | 1.052 ± 0.289 | 1:10,667 ± 8,507 | 1:1,867 ± 2,286 | 0.749 ± 0.492 | 1:<400 ± 0 | 1:<400 ± 0 | 0.003 ± 0.023 |
Day 7 post-challenge | 1:56,533 ± 39,331 | 1:15,200 ± 9,149 | 1.757 ± 0.459 | 1:34,133 ± 19,271 | 1:10,133 ± 4,253 | 1.553 ± 0.717 | 1:3,360 ± 5,311 | 1:800 ± 490 | 0.001 ± 0.007 |
Day 14 post-challenge | 1:90,667 ± 70,390 | 1:32,533 ± 21,648 | 2.011 ± 0.748 | 1:72,533 ± 65,601 | 1:16,000 ± 7,838 | 1.659 ± 0.691 | 1:71,680 ± 28,043 | 1: 8,640 ± 5,724 | 0.378 ± 0.307 |
Day 21 post-challenge | 1:78,933 ± 73,493 | 1:21,333 ± 17,013 | 1.482 ± 0.656 | 1:59,733 ± 71,802 | 1:17,067 ± 17,013 | 1.338 ± 0.675 | 1:184,320 ± 133,513 | 1:46,080 ± 33,378 | 1.294 ± 0.594 |
Day 28 post-challenge | 1:36,267 ± 17,013 | 1: 9,067 ± 4,253 | 1.867 ± 1.013 | 1:14,933 ± 5,226 | 1:4,267 ± 1,652 | 1.374 ± 0.609 | 1:307,202 ± 289,635 | 1:97,280 ± 68,692 | 1.232 ± 0.417 |