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. Author manuscript; available in PMC: 2018 May 21.
Published in final edited form as: Cell Rep. 2018 Apr 10;23(2):652–665. doi: 10.1016/j.celrep.2018.03.068

Figure 5. TH-FLP Transgenes for Restricted DA Neuron Expression.

Figure 5

(A and B) Whole-mount brain immunostaining of TH-FLP, TH-GAL4>UAS-FRT-stop-FRT-CD8::GFP (A) or TH-C-FLP, TH-GAL4>UAS-FRT-stop-FRT-CD8::GFP (B) with anti-TH (magenta), anti-GFP (green), and anti-nc82 (blue). Anterior and posterior stacks are shown, and dashed ellipses indicate DA neuron clusters as indicated.

(C) Matrix summarizing individual DA neurons labeled by different TH-FLP-based intersectional combinations. Because these expression patterns were sufficiently sparse, annotation of individual DA neurons was possible without brain registration.

(D–F) Whole-mount brain immunostaining of TH-FLP>UAS-FRT-stop-FRT-CD8::GFP in combination with R36A10-GAL4 (D), R91E12-GAL4 (E), or R92G05-GAL4 (F) with anti-TH (magenta), anti-GFP (green), and anti-nc82 (blue).

Labeled DA neurons are indicated by arrowheads. Scale bars, 100 μm.