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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: J Immunol. 2017 Dec 27;200(3):909–914. doi: 10.4049/jimmunol.1700156

Figure 1.

Figure 1

Both high and low affinity Tregs contribute to protection during autoimmunity. (A) An example of a two-TCR retrogenic chimera experimental group where Foxp3+ Treg development is limited to 4–8 TCR expressing T cells. (B) Representative flow plots of Foxp3+ Tregs from the spleen of 4–8/12-4.4m1 two-TCR Rg BM chimeras. Analysis is gated on CD4+CD3+ cells, Vβ12+ (blue) are 12-4.4m1, and Vβ12– (red) are 4–8 T cells. Mice were analyzed 5.3 weeks post-bone marrow transfer. (C and D) Diabetes incidence for two-TCR Rg chimeras expressing either 4–8 and 12-4.4m1 (C) or 1–10 and 8-1.1 TCRs (D). Mice were monitored for spontaneous diabetes development for 20 weeks (n=10–13 mice per group, #p=0.057). Data are pooled from six (C) and nine (D) independent experiments.