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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: J Immunol. 2017 Dec 27;200(3):909–914. doi: 10.4049/jimmunol.1700156

Figure 2.

Figure 2

Treg frequencies are regulated by TCR intrinsic mechanisms and are not affected by changing the size of the regulatory compartment. (A) Total Foxp3+ Treg frequencies in the spleens and islets of 4–8/12-4.4m1 two-TCR Rg BM chimeras. Analysis is gated on CD4+CD3+ (n=13–20 mice per group). (B) Frequencies of 4–8 or 12-4.4m1 Foxp3+ T cells in the spleens and islets of two-TCR Rg BM chimeras. Analysis is gated on CD4+CD3+Vβ2+ or Vβ12+ (n=13–20 mice per group). (C) Relative frequencies of 4–8 or 12-4.4m1 Foxp3– Teff cells in the islets of two-TCR Rg BM chimeras. Analysis is gated on CD4+CD3+Foxp3–Vβ2+ or Vβ12+ (n=13–20 mice per group). Mice were analyzed 5–6.5 weeks post-bone marrow transfer. Data are pooled from six independent experiments.