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Hematopoietic enhancing effect of MK and OB. (A) Experimental design of LSK, OB, and MK co-culture. Fresh OB, OB matured in vitro for 1 week (1wkOB), OB+MK co-cultured for 1 week (1wkOB+MK), and fresh MK were co-cultured with fresh LSK for 1 week, LSK were also cultured alone for 1 week, as controls. (B) Progeny were assayed for Lin−Sca1+ cells or (C) CFU, *P < 0.05 compared to fresh OB+LSK, N.S., not significantly different from fresh OB+LSK, n = 3. CFU, colony-forming unit.
