Biochemistry |
Annealing helicase? |
Yes |
Yes |
? |
Fork remodeling activities |
Fork reversal, Fork restoration |
Fork reversal, Fork restoration |
Fork reversal |
D loop activities |
D loop dissolution |
D loop dissolution, D loop inhibition |
D loop formation |
Other enzymatic activities |
|
ATP-dependent endonuclease |
Ubiquitin ligase |
Protein Structure |
Substrate recognition domain (SRD) |
HARP |
HARP-like? |
HIRAN |
SRD DNA preference |
Splayed arm junction |
Splayed arm junction |
ssDNA with 3′-OH |
Replication fork recruitment mechanism |
RPA |
Polyubiquitinated PCNA |
? |
Evolutionary conservation |
Metazoans, Bacteriophage |
Metazoans |
Metazoans to yeast |
In cells |
Reported locations of function |
Telomeres |
? |
? |
Loss of function replication phenotypes |
dsDNA breaks, fork restart deficiency |
SCEs, fork restart deficiency |
faster fork elongation with mild stress |
Drug sensitivity |
HU, CPT, MMC, IR, aphidicolin |
HU, CPT, MMS, MMC, IR, cisplatin |
UV, MMS |
Human disease |
Schimke Immunooseous Dysplasia |
endometrial cancer? |
colorectal cancer |