Table 1.

Properties of SMARCAL1, ZRANB3, and HLTF.

		SMARCAL1	ZRANB3	HLTF
Biochemistry	Annealing helicase?	Yes	Yes	?
	Fork remodeling activities	Fork reversal, Fork restoration	Fork reversal, Fork restoration	Fork reversal
	D loop activities	D loop dissolution	D loop dissolution, D loop inhibition	D loop formation
	Other enzymatic activities		ATP-dependent endonuclease	Ubiquitin ligase
Protein Structure	Substrate recognition domain (SRD)	HARP	HARP-like?	HIRAN
	SRD DNA preference	Splayed arm junction	Splayed arm junction	ssDNA with 3′-OH
	Replication fork recruitment mechanism	RPA	Polyubiquitinated PCNA	?
	Evolutionary conservation	Metazoans, Bacteriophage	Metazoans	Metazoans to yeast
In cells	Reported locations of function	Telomeres	?	?
	Loss of function replication phenotypes	dsDNA breaks, fork restart deficiency	SCEs, fork restart deficiency	faster fork elongation with mild stress
	Drug sensitivity	HU, CPT, MMC, IR, aphidicolin	HU, CPT, MMS, MMC, IR, cisplatin	UV, MMS
	Human disease	Schimke Immunooseous Dysplasia	endometrial cancer?	colorectal cancer