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. Author manuscript; available in PMC: 2018 May 21.
Published in final edited form as: Methods Mol Biol. 2016;1382:409–428. doi: 10.1007/978-1-4939-3271-9_29

Figure 3.

Figure 3

Zeroing the AP slide bar. Prior to securing the micromanipulator on the animal’s head frame in the surgery room, the AP slide bar must be zeroed out by setting the AP slide bar to a pre-determined number (example, 50) and lining up the tip of the needle/cannula with the point structure on the AP zeroing plate. When the micromanipulator is placed into the exact position on the AP bar of the head frame, the needle tip will be located at the earbars. Using a T-square when zeroing the AP slide bar helps to ensure that the needle is straight.