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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Mutat Res. 2018 Mar 20;809:6–12. doi: 10.1016/j.mrfmmm.2018.03.002

Figure 2.

Figure 2

Characterization of purified monoadducts. Panel A) Lane 1: purified cisplatin monoadducts; Lane 2: purified DEB monoadducts; Lane 3: purified ECH monoadducts; Lane 4: unmodified single-stranded DNA; Lane 5: piperidine-treated cisplatin monoadducts; Lane 6: piperidine-treated DEB monoadducts; Lane 7: piperidine-treated ECH monoadducts: Lane 8: G-reaction. Monoadducts were several days old. Alkylation, but not platination, at the N7-position results in cleavage at the single G residue of Strand 1. Panel B) Lane 1: G-reaction; Lane 2: unmodified single-stranded DNA; Lane 3: DMS- and piperidine-treated cisplatin monoadduct. Cisplatin protects from DMS attack, verifying linkage at the N7 of guanine.