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Primer (14mer) extension by hPol β. Strand 1 was unmodified or modified with cisplatin, DEB, or ECH. Incubation was at 37°C for the indicated time points. The band in the t= 0 drug-treated lanes with lower mobility than the 22mer corresponds to monoadducted Strand 1 (“Mono”). Control 14mer and full-length 22mer are in the first and last lane, respectively. The sequence of the newly synthesized strand is shown along the right margin.
