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Single nucleotide addition with KF. Strand 1, either unmodified or monoadducted, was first annealed with primer. The first lane in each set is the no-dNTP control. The 13mer primer reveals nucleotide addition one nucleotide before the adduct (across from T); the 14mer reflects addition across from the adduct (at G), and the 15mer reflects addition one nucleotide after the adduct (across from C). (A) Cisplatin-adducted template; (B) DEB-adducted template; (C) ECH-adducted template.
