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Steady-state kinetic parameters for primer extension by hPol β.
| Template | kcat (min−1) | Km (dNTP)(μM) | kcat/Km (1/min-μM) | Relative Efficiencya |
|---|---|---|---|---|
| Unmodified | 6.3 ± 1.1 | 111 ± 77 | 0.057 | 1.0 |
| Cisplatin monoadduct | 0.23 ± 0.22 | 48 ± 25 | 0.0048 | 0.084 |
| DEB monoadduct | 0.31 ± 0.16 | 42 ± 32 | 0.0074 | 0.13 |
| ECH monoadduct | 0.38 ± 0.24 | 51 ± 55 | 0.0075 | 0.13 |
The ratio of kcat/Km of the monoadduct-containing template versus the unmodified template.
