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. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: Cell Biol Int. 2017 Dec 7;42(6):630–642. doi: 10.1002/cbin.10913

Figure 1. Growth properties of mrpl34/ts mutants.

Figure 1

(A) Comparative growth of wild-type cells (wt) and mrpl34 null mutant alone (Δmrpl34), or harboring plasmids expressing different mrpl34 mutants: ts alleles ts1, ts2 and ts3 (Δmrpl34/ts1, Δmrpl34/ts2, Δmrpl34/ts3(R95W) and the point mutants F79A (Δmrpl34/F79A) W101R (Δmrpl34/W101R) as well as an empty plasmid (Δmrpl34/vector) and a plasmid with the wild-type MRPL34 gene (Δmrpl34/MRPL34). Cultured cells were serially diluted and spotted in rich glucose media (YPD) and rich ethanol-glycerol media (YPEG) plates. Plates were incubated at 30° C and 37° C as indicated and photographed after three days of incubation. (B) Comparative growth of the point mutants F79A (Δmrpl34/F79A) and W101R (Δmrpl34/W101R) was also performed as in “A”.