Table 1.
Bacterial strains and plasmids used in this study
| Genotype/characteristics | Source | |
|---|---|---|
| Strains | ||
| Top10 | F− mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (StrR) endA1 nupG | Invitrogen |
| BL21(DE3)pLysS | F− ompT hsdSB(r−B m−B) gal dcm (DE3) pLysS (CmR) | Novagen |
| Rosetta(DE3)pLysS | F− ompT hsdSB(r−B m−B) gal dcm lacY1 (DE3) pLysSRARE (CmR) | Novagen |
| Plasmids | ||
| pET-21a | Ampr T7 promoter lac operator | Novagen |
| pUCm-T | TA cloning | Sangon |
| pT-RFP | pUCm-T carrying mcherry | This study |
| pET-RFP | pET-21a expressing mCherry | This study |
| pO-RFP | pET-21a carrying mcherry in ORF2 | This study |
| pOI-RFP | pO-RFP with one rare codon in ORF1 | This study |
| pOII-RFP | pO-RFP with two consecutive rare codons in ORF1 | This study |
| pOIII-RFP | pO-RFP with three consecutive rare codons in ORF1 | This study |
| pT-GFP | pUCm-T carrying egfp | This study |
| pGFP-RFP | pO-RFP carrying egfp in ORF1 | This study |
| pGFPI-RFP | pGFP-RFP with one rare codon preceding egfp | This study |
| pGFPII-RFP | pGFP-RFP with two consecutive rare codons preceding egfp | This study |