Fig. 1.
Inhibition of Bacillus subtilis BS168 and Escherichia coli TG1 by Bacillus sp. GFP-2. Activated Bacillus subtilis BS168 (Gram-positive, OD600nm = 1.0) (A) and Escherichia coli TG1 (Gram-negative, OD600nm = 1.0) (B) were inoculated as target bacteria into LB agar plates. Sterilized oxford cups were put on pre-coating LB agar plates and 250 μl of LB medium (a, negative control), supernatant (d) and related thallus precipitate (c) of strains GFP-2 (OD600nm = 2.0), or ampicillin (1 mg/ml) (b, positive control), were added onto the plates, and the plates were incubated at 37 °C for 12 h. Overnight cultured BS168 (OD600nm = 3.28) (C) and TG1 (OD600nm = 1.86) (D) were inoculated, respectively, into 5 ml of liquid LB culture media at the ratio of 1:100, in the presence of control medium (diamond), 25 µg/ml ampicillin (square), or GFP-2 culture media (100 µl for TG1, 500 µl for BS168) (triangle), and cultured at 37 °C with shaking at 220 rpm. 0, 1, 2, 3, 4, 5, 6, 8, 10 and 12 h after the inoculation, the growth of bacteria was monitored using optical density (O.D) method at 600 nm