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. 2018 May 22;8:84. doi: 10.1186/s13568-018-0614-3

Fig. 2.

Fig. 2

The phylogenetic relationship of Bacillus sp. GFP-2 and related strains. The 16S rRNA sequence of Bacillus sp. GFP-2 was amplified by PCR and was identified using EzBiocloud service. a Phylogenetic trees were reconstructed by the neighbor-joining methods with the MEGA 5 program package. Evolutionary distances were calculated according to the algorithm of the Kimura two-parameter model. b OrthoANI values and Unweighted Pair Group Method with Arithmetic Mean (UPGMA) trees between strain GFP-2 and closely related strains were analyzed by Orthologous Average Nucleotide Identity Tool (OAT)