Table 1.

Summary of all conditions used to prepare siRNA and their subsequent effect upon siRNA uptake efficiency in 3D culture.

siRNA complexes formed in:	Matrigel penetration	Organoid uptake
Opti-MEM + Lipofectamine RNAiMAX	✗	✗
Opti-MEM + Lipofectamine 2000	✗	✗
Opti-MEM formed siRNA into well containing 10% serum	✓✓	✓✓
10% serum formed siRNA into well containing Opti-MEM	✓✓	✓✓
10% serum	✓✓	✓✓
1% serum	✓	✓
0.1% serum	✗	✗
10% BSA	✓	✗
1% BSA	✓	✗
0.1% BSA	✗	✗
DMEM only	✗	✗
3D organoid medium	✗	✗
10% dialyzed serum	✓✓	✓✓
1% dialyzed serum	✓	✓
0.1% dialyzed serum	✗	✗