Figure 2.

Specific-allergen Immunotherapy reduces ERs in Der f-sensitized mice. (a) The expression of BIP and CHOP was measured using immune staining. DAPI was used for staining nucleus. (b,c) Fluorescence intensity of BIP and CHOP at airway location was measured 10 times at random fields in each group. (d) Expression of p-PERK, PERK, p-eIF2α, BIP, CHOP in lung tissue were determined by western blot. (e–g) Relative changes in the density of BIP, CHOP and β-actin, p-PERK and PERK were analysed. Blots in panel (d) have been cropped, and full blots are presented in Supplementary Fig. S2. All data were represented as means ± SEM (n = 6, one-way ANOVA, significant differences were defined as p < 0.05).