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. 2018 May 21;8:7950. doi: 10.1038/s41598-018-26221-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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IL-25 induces ERs in 16HBE cells. (a) Expression of BIP and CHOP were measured by immunofluorescent staining with BIP and CHOP antibody. DAPI was used for staining nucleus. TRICT-conjugated secondary abs (red) and FITC-conjugated secondary abs (green) were used for binding confocal laser scanning microscopy (original magnification, ×400). Fluorescence intensity of BIP (b) and CHOP (c) were quantified at 10 random fields. The mRNA levels of CHOP induced by IL25 were detected at a dose-dependent manner (d) (0 ng/ml, 1 ng/ml, 10 ng/ml, 100 ng/ml) and a time-dependent manner (e) (0 h, 4 h, 12 h, 24 h). All data were represented as means ± SEM (n = 6, one-way ANOVA with Tukey’s post hoc, significant differences were defined as p < 0.05).

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