Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 May 21;8:7950. doi: 10.1038/s41598-018-26221-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

4-PBA inhibits IL-25-induced epithelial barrier damage and suppresses apoptosis of 16HBE cells associated with PERK pathway. (a) The apoptotic cells were determined by flow cytometry using Annexin V/Propidium iodide (PI) staining. (b) The percentages of apoptotic cells were analysed. (c–f) Confocal laser immunofluorescence photomicrographs of ZO-1 and E-cadherin were measured (original magnification, ×400). Epithelial barrier function was detected by TEER measurement (g) and FITC-DX paracellular flux assay (h,i) The expression of p-PERK, PERK, p-eIF2α, BIP and CHOP were measured using immunoblotting. Relative changes in the density of BIP (j), CHOP (k) and β-actin, p-PERK and PERK (l) were analysed. Blots in panel (g) have been cropped, and full blots are presented in Supplementary Fig. S5. Data were represented as means ± SEM (n = 6, one-way ANOVA with Tukey’s post hoc, significant differences were defined as p < 0.05).

HHS Vulnerability Disclosure