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. 2018 May 21;8:7918. doi: 10.1038/s41598-018-26343-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of acrylamide exposure on the adult zebrafish brain proteome. (a) Unsupervised heat map of the differential proteins. Hierarchical euclidean clustering was performed on the intensities of the differential proteins after Z-score normalization. It was done using the Perseus software. Left panel represents the down-regulated proteins and the right panel the up-regulated proteins following the ACR treatment. (b) ACR-modified cysteine residues. Left panel represent the expression levels of the 191 proteins containing ACR-modified cysteine residues, and the right panel the Log-2 intensities of the peptides with ACR –adducts in cysteine residues. CN: control pool (n = 5 pools; 3 brains each); ACR: pool ACR (n = 5 pools; 3 brains each).