Table 3.
The characteristics of L-asparaginase (wild-type) and its mutations.
| Enzyme | Specific activity (U/mg) | Fold | t(1/2,85°C) (min) | Optimum temperature (°C) | Optimum pH |
|---|---|---|---|---|---|
| Wild-type | 1483.81 ± 63.61 | 1 | 105 ± 5 | 95 | 8.0 |
| A1 | 2314.50 ± 93.12 | 1.56 | 105 ± 5 | 95 | 8.0 |
| B1 | 2145.41 ± 78.36 | 1.45 | 140 ± 5 | 95 | 8.0 |
| C1 | 1633.41 ± 83.15 | 1.10 | 100 ± 5 | 95 | 8.0 |
| D2 | 2687.4 ± 84.11 | 1.81 | 110 ± 5 | 95 | 8.0 |
| E2 | 2899.12 ± 97.35 | 1.95 | 150 ± 5 | 95 | 8.0 |
| F2 | 2490.50 ± 65.44 | 1.68 | 100 ± 5 | 95 | 8.0 |
| S17G | 2556.25 ± 88.12 | 1.72 | 110 ± 5 | 95 | 8.0 |
| A90S | 1462.3 ± 75.33 | 0.99 | 150 ± 5 | 95 | 8.0 |
| R156S | 2414.33 ± 68.55 | 1.62 | 105 ± 5 | 95 | 8.0 |
| K272A | 1889.32 ± 59.9 | 1.27 | 100 ± 5 | 95 | 8.0 |
| S17G/A90S/R156S/K272A | 3108.43 ± 105.3 | 2.10 | 145 ± 5 | 95 | 8.0 |
The wild-type enzyme in this study was the Pyrococcus yayanosii CH1 L-asparaginase.