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. 2018 May 15;9:975. doi: 10.3389/fimmu.2018.00975

Figure 2.

Figure 2

Early leukocyte recruitment to the airways and neutrophil recruitment to the lungs during influenza A virus infection are more pronounced in phosphatidyl inositol 3 kinase-gamma (PI3Kγ) knockout (KO) mice. WT and PI3Kγ KO mice were infected with WSN or phosphate-buffered saline (Mock control) and killed at days 3 and 5 after infection (n = 4–8 mice per group and time point). (A) Bronchoalveolar lavage was performed to assess recruitment of total leukocytes (red columns), macrophages (orange columns), neutrophils (yellow columns), and lymphocytes (blue columns) to the airways in cytospin stained slides. (B) Recruitment of neutrophils to the lungs was assessed by myeloperoxidase assay. Cell numbers are presented as mean ± SD. *p < 0.05, **p < 0.01, and ***p < 0.001, comparing to Mock or indicated groups and #p < 0.05, ##p < 0.01, and ###p < 0.001, when compared with WT control in the same time point (one-way ANOVA, Newman–Keuls).