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. 2018 May 15;9:975. doi: 10.3389/fimmu.2018.00975

Figure 4.

Figure 4

Phosphatidyl inositol 3 kinase-gamma (PI3Kγ) activation on bone marrow-derived macrophages (BMDM) leads to interferon-stimulated gene 15 (ISG15) production during influenza A virus infection in a p38-dependent way. BMDM from WT and PI3Kγ knockout (KO) were infected with influenza WSN [multiplicity of infection (MOI): 3.0]. (A) One hour after infection, BMBM protein extracts were analyzed for p38 phosphorylation; β-actin was a loading control. (B) WT BMDM were pretreated with the p38 inhibitor SB203580 or vehicle and KO BMDM were treated with vehicle then infected with WSN (MOI: 3.0). ISG15 protein expression was assessed at 6 h after infection; β-actin was a loading control. Results are presented as mean ± SD. **p < 0.01, when comparing WT-infected cells treated or not with SB203580; ##p < 0.01, comparing PI3Kγ KO to WT infected BMDM (one-way ANOVA, Newman–Keuls).